A Handbook of Gene and Cell Therapy by Clévio Nóbrega & Liliana Mendonça & Carlos A. Matos
Author:Clévio Nóbrega & Liliana Mendonça & Carlos A. Matos
Language: eng
Format: epub
ISBN: 9783030413330
Publisher: Springer International Publishing
4.2.3 Intracellular Trafficking
After escaping the endosome, the vectors must deliver their genetic material to the nucleus. This intracellular transport barrier is also important, especially for non-viral vectors, as they must ensure the trafficking to the nucleus and protect the genetic material from degradation by cytoplasmic nucleases. The incorporation of specific molecules such as polyethylenimine (PEI) in non-viral vectors allows safe intracellular transport of the DNA cargo through the microtubules to the nucleus.
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